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You are here:Home » Antibodies » Abs to Neuron Specific Enolase » Anti -Neuron Specific Enolase (NSE, Gamma-enolase, 2-phospho-D-glycerate Hydro-lyase, Neural Enolase, Enolase 2, ENO2)

Anti -Neuron Specific Enolase (NSE, Gamma-enolase, 2-phospho-D-glycerate Hydro-lyase,
Neural Enolase, Enolase 2, ENO2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH
NSE (neuron specific enolase), also known as Enolase 2 (ENO2), is found in elevated concentrations in plasma in certain neoplasias. The enolases catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. ENO2 gene contains 12 exons distributed over 9,213 nucleotides. Human neurone-specific enolase is mapped to chromosome 12p13.
Catalog #N2175-01L
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 0.1-0.5ug/ml
Immunohistochemistry (paraffin): 1-2ug/ml Heat-mediated antigen retrieval is recommended.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to aa 420-434, EARFAGHNFRNPSVL, from thr C-terminus of human NSE.
SpecificityRecognizes human NSE. Species Crossreactivity: mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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