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You are here:Home » Antibodies » Abs to Neuron Specific Enolase » Anti -Neuron Specific Enolase (NSE, 2-phospho-D-glycerate Hydrolyase, Enolase 2, Enolase-2, Eno2, ENOG, Enolase 2 (gamma, neuronal), Gamma Enolase, Neural Enolase, Neuron Specific gamma Enolase)

Anti -Neuron Specific Enolase (NSE, 2-phospho-D-glycerate Hydrolyase, Enolase 2,
Enolase-2, Eno2, ENOG, Enolase 2 (gamma, neuronal), Gamma Enolase, Neural Enolase, Neuron
Specific gamma Enolase)

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Specifications

Clone Host Grade Applications
Polyclonal Chicken Affinity Purified IH IC
Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma and certain neoplasias. These include pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.
Catalog #N2175-02L
Positive Control Mouse brain (see photo)
ApplicationsSuitable for use in Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostChicken
SourceMouse
Concentration~0.2mg/ml
FormAs reported
PurityPurified by immunoaffinity chromatography.
ImmunogenTwo different affinity-purified anti-peptide antibodies were combined to make this product. The concentrations of each of the two antibodies was 100ug/ml (based on Bradford assay readings using bovine serum albumin as a standard), making the total antibody concentration 200ug/ml.
SpecificitySpecies Crossreactivity: Reacts with mouse NSE type 2. Other species have not been tested. Homology with human is 100% for both peptides.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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