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You are here:Home » Antibodies » Abs to Neuron Specific Enolase » Anti -Neuron Specific Enolase (NSE)

Anti -Neuron Specific Enolase (NSE)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH
Catalog #N2175-04
Enolases are homo- or heterodimers of the three subunits alpha (46kD), beta (44kD), and gamma (46kD). The alpha-subunit is expressed in most tissues and the beta-subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.
ApplicationsSuitable for use in ELISA and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (paraffin): 1:100-1:300
Optimal dilution must be determined by the user.
Matched PairCapture: N2175-04
Detection: N2175-06
Affinity Constant1x10e-8 - 1x10e-9 L/mole
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No1.B.648
HostMouse
SourceHuman
Concentration~3mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityProtein by Protein G affinity chromatography.
ImmunogenPurified neuron-specific enolase from human brain.
SpecificityRecognizes gamma enolase subunit without detectable crossreactivity with the alpha or beta subunits. Reacts with 45kD band corresponding to NSE in immunoblotting of human brain extracts.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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