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You are here:Home » Antibodies » Abs to Neuron Specific Enolase » Anti -Neuron Specific Enolase (NSE)

Anti -Neuron Specific Enolase (NSE)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH
Catalog #N2175-05
Enolases are homo- or heterodimers of the three subunits alpha (46kD), beta (44kD), and gamma (46kD). The alpha-subunit is expressed in most tissues and the beta-subunit only in muscle. The gamma-subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.
ApplicationsSuitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunoprecipitation: 2ug/mg protein lysate. Native and denatured. Use Protein G.
Western Blot: 1-2ug/ml for 2 hours at RT.
Immunohistochemistry (Formalin/Parafin): 1:800-1:1600 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive ControlIMR5 cells, pancreas.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No0.N.468
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide, before the addition of glycerol to 40%.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified human Neuron Specific Enolase. Cellular Localization
SpecificityRecognizes human neuron-specific enolase (NSE). Shows no crossreactivity with the alpha or beta subunits of NSE.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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