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You are here:Home » Antibodies » Abs to Neuron Specific Enolase » Anti -Neuron Specific Enolase, gamma (gNSE)

Anti -Neuron Specific Enolase, gamma (gNSE)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IH
Catalog #N2175-07
Enolases are homo- or heterodimers of the three subunits alpha (46kD), beta (44kD) and gamma (46kD). The alpha-subunit is expressed in most tissues. The beta-subunit is expressed only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms.
ApplicationsSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: Formalin-fixed and paraffifn-embedded sections.
Optimal dilutions to be determined by the researcher.
Matched PairsCapture: N2175-06, N2175-07
Detection: N2175-07, N2175-06
HybridomaSp2/0 myeloma cells with spleen cells from Balb/c mice.
Source Ascites
Affinity Constant1x10e-8 to 1x10e-9
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No1.B.650
HostMouse
SourceHuman
Concentration~8mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography ( 95%). Purity is tested by electrophoresis.
ImmunogenPurified neuron-specific enolase from human brain.
SpecificityGamma enolase. Specific for the gamma subunit without detectable crossreactivity with the alpha or beta subunits. Reacts with 45kD band corresponding to NSE in immunoblotting of human brain extracts.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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