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You are here:Home » Antibodies » Antibodies-Neuron Specific Enolase » Anti -Neuron-Specific Enolase (NSE), type 2

Anti -Neuron-Specific Enolase (NSE), type 2


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Clone Host Grade Applications
Polyclonal Chicken Affinity Purified B IH IC
Human NSE-2 (EC is a 47.138 kD protein (434aa) expressed in neurons of the peripheral nervous system (PNS) and central nervous system (CNS). NSE-2 catalyzes the conversion of 2-phospho-D-glycerate into phosphoenol pyruvate, and is an essential enzyme in energy metabolism in nervous tissues.
Catalog #N2175-10B
ApplicationsSuitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: 1:2000-1:5000 using 2% paraformaldehyde tissues
Immunocytochemistry: 1:2000-1:5000 using 2% paraformaldehyde cells
Western Blot: 1:5000-1:10,000
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1906 IgY, Chicken Pab Rb x Ch
I1906-10 IgY, Chicken (HRP) Rb x Ch
I1906-12 IgY, Chicken (FITC) Rb x Ch
I1906-14 IgY, Chicken (AP) Rb x Ch
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenTwo synthetic peptides corresponding to different regions of the Neuron-Specific Enolase, type-2 gene product (KLH). Species sequence homology: human (NP_001966, NCBI) and rat (AAA41119, NCBI)
SpecificityRecognizes Neuron-Specific Enolase type 2. Species crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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