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You are here:Home » Antibodies » Abs to Neuron Specific Enolase » Anti -Neuron-Specific Enolase (NSE), type 2

Anti -Neuron-Specific Enolase (NSE), type 2

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Specifications

Clone Host Grade Applications
Polyclonal Chicken Affinity Purified B IH IC
Human NSE-2 (EC 4.2.1.11) is a 47.138 kD protein (434aa) expressed in neurons of the peripheral nervous system (PNS) and central nervous system (CNS). NSE-2 catalyzes the conversion of 2-phospho-D-glycerate into phosphoenol pyruvate, and is an essential enzyme in energy metabolism in nervous tissues.
Catalog #N2175-10B
ApplicationsSuitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: 1:2000-1:5000 using 2% paraformaldehyde tissues
Immunocytochemistry: 1:2000-1:5000 using 2% paraformaldehyde cells
Western Blot: 1:5000-1:10,000
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1906 IgY, Chicken Pab Rb x Ch
I1906-10 IgY, Chicken (HRP) Rb x Ch
I1906-12 IgY, Chicken (FITC) Rb x Ch
I1906-14 IgY, Chicken (AP) Rb x Ch
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgY
HostChicken
SourceMouse
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenTwo synthetic peptides corresponding to different regions of the Neuron-Specific Enolase, type-2 gene product (KLH). Species sequence homology: human (NP_001966, NCBI) and rat (AAA41119, NCBI)
SpecificityRecognizes Neuron-Specific Enolase type 2. Species crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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