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Specifications

Clone Host Grade Applications Polyclonal Sheep Serum IH RIA

Catalog #	N2176-60
Applications	Suitable for use in Immunohistochemistry and RIA. Synthetic NPY completely abolishes radio-labeled NPY binding to NPY antibody in RIA and antibody binding to tissue antigen. Other applications not tested.
Recommended Dilution	Immunohistochemistry: 1:5000 on rabbit and guinea pig using an ABC detection method.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Isotype	IgG
Host	Sheep
Source	Bovine
Concentration	Not Determined
Form	Supplied as a lyophilized powder. No preservatives added. Reconstitute with 100ul of sterile 40-50% glycerol, ddH2O..
Purity	Serum
Immunogen	Synthetic NPY peptide conjugated to bovine thyroglobulin. Human Peptide YY shares 65% homology amino acid sequence for human NPY.
Specificity	Recognizes bovine Neuropeptide Y (NPY).
Synthetic Peptide Crossreactivity	Somatostatin-14, leu-enkephalin, met-enkephalin, oxytocin, Agr8-vasopressin, vasoactive intestinal peptide, angiotensin II
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.