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Anti -Neutrophils (Ly-6G NEUTROPHIL MARKER, Gr1, Gr-1, Ly-6G, Ly-6G.1, Lymphocyte antigen 6G precursor, Lymphocyte antigen Ly-6G.1 precursor) (Biotin)
Anti -Neutrophils (Ly-6G NEUTROPHIL MARKER, Gr1, Gr-1, Ly-6G, Ly-6G.1, Lymphocyte antigen
6G precursor, Lymphocyte antigen Ly-6G.1 precursor) (Biotin)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rat |
Affinity Purified |
E IH FC |
|
| Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G is a GPI- anchored protein and a good marker of peripheral neutrophils. Although predominantly present on neutrophils, it is also expressed on a subset of eosinophils, differentiating pre-monocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells and T cell subsets. N2254-01 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has an inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days. | | | Catalog # | N2254-01 | | Applications | Suitable for use in ELISA, Flow Cytometry, Functional Studies and Immunohistochemistry. Other applications not tested. | | Recommended Dilutions | Flow Cytometry: 1:50 (10ug/ml) labels 5x10e5 cells (4) | | Immunohistochemistry (Frozen): 1:50 acetone fixation is recommended. (7) | | Immunohistochemistry (Paraffin): 1:50 blocking with 20% normal rabbit serum. (5) | | Functional Studies (remove sodium azide): Neutrophil depletion. Mice were tested with N2254-01 given intraperitoneally at a dose of 1mg, 6 hours before injection. (6) | | Optimal dilutions to be determined by the researcher. | | Recommended Positive Control | Mouse neutrophils | | Recommended Negative Control | Mouse Thymocytes | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 6A608 | | Host | Rat | | Source | Mouse | | Concentration | ~0.1mg/ml | | Form | Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide. Labeled with Biotin. | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Purified BALB/c mouse neutrophils | | Specificity | Recognizes mouse Ly-6G and Ly-6C. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Ly-6G/Ly-6C |
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