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You are here:Home » Antibodies » Abs to Neutrophils » Anti -Neutrophils (Ly-6G NEUTROPHIL MARKER, Gr1, Gr-1, Ly-6G, Ly-6G.1, Lymphocyte antigen 6G precursor, Lymphocyte antigen Ly-6G.1 precursor) (Biotin)

Anti -Neutrophils (Ly-6G NEUTROPHIL MARKER, Gr1, Gr-1, Ly-6G, Ly-6G.1, Lymphocyte antigen
6G precursor, Lymphocyte antigen Ly-6G.1 precursor) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E IH FC
Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G is a GPI- anchored protein and a good marker of peripheral neutrophils. Although predominantly present on neutrophils, it is also expressed on a subset of eosinophils, differentiating pre-monocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells and T cell subsets. N2254-01 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has an inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.
Catalog #N2254-01
ApplicationsSuitable for use in ELISA, Flow Cytometry, Functional Studies and Immunohistochemistry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:50 (10ug/ml) labels 5x10e5 cells (4)
Immunohistochemistry (Frozen): 1:50 acetone fixation is recommended. (7)
Immunohistochemistry (Paraffin): 1:50 blocking with 20% normal rabbit serum. (5)
Functional Studies (Remove sodium azide): Neutrophil depletion. Mice were tested with NIMP-R14 given intraperitoneally at a dose of 1mg, 6 hours before injection. (6)
Optimal dilutions to be determined by the researcher.
Recommended Positive Control Mouse neutrophils
Recommended Negative Control Mouse Thymocytes
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No6A608
HostRat
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide. Labeled with Biotin.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified BALB/c mouse neutrophils
SpecificityRecognizes mouse Ly-6G and Ly-6C.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesLy-6G/Ly-6C


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