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You are here:Home » Antibodies » Antibodies-Neutrophils » Anti -Neutrophils (Ly-6G NEUTROPHIL MARKER, Gr1, Gr-1, Ly-6G, Ly-6G.1, Lymphocyte antigen 6G precursor, Lymphocyte antigen Ly-6G.1 precursor) (FITC)

Anti -Neutrophils (Ly-6G NEUTROPHIL MARKER, Gr1, Gr-1, Ly-6G, Ly-6G.1, Lymphocyte antigen
6G precursor, Lymphocyte antigen Ly-6G.1 precursor) (FITC)


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Clone Host Grade Applications
Monoclonal Rat Affinity Purified IH FC
The Ly-6G locus encodes for a family of Ly-6 proteins including Ly-6G. This protein is expressed on mouse neutrophils, a subset of eosinophils, a differentiating pre-monocytes and plasmacytoid dendritic cells. The monoclonal antibody NIMP-R14 has been succesfully used to stain neutrophils for fluorescent activated cell sorting and in frozen and paraffin sections. Furthermore it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.
Catalog #N2254-01A
ApplicationsSuitable for use in FLISA, Flow Cytometry, Immunohistochemistry and Functional studies. Other applications not tested.
Recommended DilutionsFlow Cytometry: Incubate 5x10e5 cells with 10ug/ml antibody (ref 4)
Immunohistochemistry (Paraffin): 1:50 Block with 20% normal rabbit serum (ref 5)
Immunohistochemistry (Frozen): 1:50 acetone fixation recommended (ref 7)
Functional Studies: Neutrophil depletion. MIce were treated with N2254-01A given intraperitoneally at a dose of 1mg, 6 hours before infection (ref 6)
Optimal dilutions to be determined by the researcher.
Recommended Positive ControlMouse neutrophils
Recommended Negative ControlMouse thymocytes
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Clone TypeMonoclonal
Clone No6A608
FormSupplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified BALB/c mouse neutrophils
SpecificityRecognizes mouse neutrophils (Ly-6G and Ly-6C).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesLy-6G/Ly-6C

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