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You are here:Home » Antibodies » Abs to Neutrophils » Anti -Neutrophils (Allotypic Marker)

Anti -Neutrophils (Allotypic Marker)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified B IH FC
Catalog #N2254-01C
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry (frozen), and Western Blot. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
HybridomaY3 Ag1.2.3 rat myeloma cells with spleen cells from AO rats.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No10F221
HostRat
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.09% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenCultured bone marrow cells.
SpecificityRecognizes a polymorphic 40kD antigen expressed by murine polymorphonuclear cells, but absent on resident tissue macrophages. Strains positive for 7/4
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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