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You are here:Home » Antibodies » Antibodies-Neutrophils » Anti -Neutrophils Ly-6B.2 (Allotypic Marker) (Biotin)

Anti -Neutrophils Ly-6B.2 (Allotypic Marker) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified FC
Ly-6B.2 is a ~25-30kD GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages (Rosas et al. 2010). High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15kD (Rosas et al.2010).
Catalog #N2254-01F
This antibody has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow (Bertoncello et al. 1991) and the depletion of myeloid cells in vivo (Rosas et al. 2010).
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: Neat-1:10; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
HybridomaY3 Ag1.2.3 rat myeloma cells with spleen cells from AO rats.
Storage and StabilityMay be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No10F229
HostRat
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with Biotin.
PurityPurified by Protein G affinity chromatography.
ImmunogenCultured bone marrow cells.
SpecificityRecognizes Ly-6B.2 Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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