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You are here:Home » Antibodies » Abs to Neutrophils » Anti -Neutrophils (Allotypic Marker) (FITC)

Anti -Neutrophils (Allotypic Marker) (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified FC
Ly-6B.2 is a ~25-30kD GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages (Rosas et al. 2010). High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15kD (Rosas et al.2010).
Catalog #N2254-01G
N2254-01G has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow (Bertoncello et al. 1991) and the depletion of myeloid cells in vivo (Rosas et al. 2010).
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionsFlow Cytometry: Neat-1:10. 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
HybridomaY3 Ag1.2.3 rat myeloma cells with spleen cells from AO rats.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No10F229
HostRat
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 1% BSA, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
PurityPurified by Protein G affinity chromatography.
ImmunogenCultured bone marrow cells.
SpecificityRecognizes the Ly-6B.2 antigen. Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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