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You are here:Home » Antibodies » Abs to Neutrophils » Anti -Neutrophils (Allotypic Marker) (PE)

Anti -Neutrophils (Allotypic Marker) (PE)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified FC
Catalog #N2254-01K
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: Neat; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
HybridomaY3 Ag1.2.3 rat myeloma cells with spleen cells from AO rats.
Storage and StabilityLyophilized powder may be stored at 4°C. Reconstitute by adding sterile buffer or ddH2O. Aliquot and store at 4°C. DO NOT FREEZE! Reconstituted product is stable for 12 months as an undiluted liquid. Dilute only prior to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No10F229
HostRat
SourceMouse
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.
PurityPurified by Protein G affinity chromatography.
ImmunogenCultured bone marrow cells.
SpecificityRecognizes a polymorphic 40kD antigen expressed by murine polymorphonuclear cells, but absent on resident tissue macrophages. Strains positive for 7/4
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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