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You are here:Home » Antibodies » Abs to Neutrophils » Anti -Neutrophils (Ly-6G NEUTROPHIL MARKER, Gr1, Gr-1, Ly-6G, Ly-6G.1, Lymphocyte antigen 6G precursor, Lymphocyte antigen Ly-6G.1 precursor) (PE)

Anti -Neutrophils (Ly-6G NEUTROPHIL MARKER, Gr1, Gr-1, Ly-6G, Ly-6G.1, Lymphocyte antigen
6G precursor, Lymphocyte antigen Ly-6G.1 precursor) (PE)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E IH FC
The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly6G is together with Ly6c a component of the myeloid differentiation antigen Gr-1. Ly6G a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating pre- monocytes and plasmacytoid dendritic cells. Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.
Catalog #N2254-01N
ApplicationsSuitable for use in Flow Cytometry, ELISA, Functional Studies and Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry: Frozen
Immunohistochemistry (Paraffin): Blocking with 20% normal rabbit serum
Flow Cytometry: 10ul/250.000 cells in a 100ul total staining volume
Functional Studies: Neutrophil depletion. Mice were treated with NIMP-R14 given intraperitoneally at a dose of 1mg, 6h before infection
Optimal dilutions to be determined by the researcher.
Positive Control Mouse neutrophils
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No13A63
HostRat
SourceMouse
ConcentrationAs reported
FormSupplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with R-Phycoerythrin (PE).
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified BALB/c mouse neutrophils
SpecificityRecognizes mouse Ly-6G and Ly-6C.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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