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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IH FC

Alpha defensins belong together with the beta-defensins to the family of cationic trisulfide-containing microbicidal peptides. They exert their microbicidal activity by permeating the membrane involving the formation of voltage regulated ion channels. Rabbit neutrophils contain large amount of alpha defensins (Neutrophil Defensin) NP 1-6. Defensins constitute more than 15% of total protein in rabbit neutrophils and are accumulated in dense cytoplasmic granules. Rabbit neutrophils express all six rabbit alpha-defensins, NP 1-6. Among myeloid rabbit alpha-defensins NP 1-6, NP 3-6 are neutrophil specific, while NP 1-2 are expressed in rabbit alveolar macrophages. Defensins are relatively resistant to proteolysis, low pH and boiling. Rabbit neutrophil defensins, whose three-dimensional structures are very similar to those of human alpha defensins may be helpful in understanding the role of antimicrobial peptides in innate and acquired immunity and in the studies on the role of neutrophils in pathological situations and in various rabbit models of human diseases.
Catalog #	N2258-25
Applications	Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:10
	Flow Cytometry: 1:10
	Immunohistochemistry (Paraffin, frozen): 1:10
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	6A609
Host	Mouse
Source	Rabbit
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Rabbit Neutrophil Defensin 5 (NP5).
Specificity	Recognizes natural, synthetic and recombinant rabbit Neutrophil Defensin 5. Reacts with natural NP-5 in biological solutions, in tissue sections and leukocyte smears fixed with ethanol, methanol/acetone or paraformaldehyde and in Flow Cytometry analysis of rabbit neutrophils stained by cell permeabilization method.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.