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You are here:Home » Molecular Biology » MB-Enzymes, Endonuclease » NTHL1, Recombinant, Human, aa1-312, His-Tag (Endonuclease III-like protein 1, NTH1, OCTS3, EC 4.2.99.18)

NTHL1, Recombinant, Human, aa1-312, His-Tag (Endonuclease III-like protein 1, NTH1, OCTS3,
EC 4.2.99.18)

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Specifications

NTHL1 has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions
Catalog #N2291-43
DescriptionRecombinant human NTHL1 protein fused to His-tag at the N-terminus; expressed in E. coli
AA SequenceMGSSHHHHHH SSGLVPRGSH MCSPQESGMT ALSARMLTRS RSLGPGAGPR GCREEPGPLR RREAAAEARK SHSPVKRPRK AQRLRVAYEG SDSEKGEGAE PLKVPVWEPQ DWQQQLVNIR AMRNKKDAPV DHLGTEHCYD SSAPPKVRRY QVLLSLMLSS QTKDQVTAGA MQRLRARGLT VDSILQTDDA TLGKLIYPVG FWRSKVKYIK QTSAILQQHY GGDIPASVAE LVALPGVGPK MAHLAMAVAW GTVSGIAVDT HVHRIANRLR WTKKATKSPE ETRAALEEWL PRELWHEING LLVGFGQQTC LPVHPRCHAC LNQALCPAAQ GL
Molecular Weight36.6kD (332aa) confirmed by MALDI-TOF
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C or lower. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight36.6kD
SourceE. coli
Purity80% (SDS-PAGE)
Concentration~0.25mg/ml (Bradford assay)
FormSupplied as a liquid in 20mM Tris-HCl buffer, pH8.0, containing 0.15M sodium chloride, 1mM DTT and 40% glycerol
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesEndonuclease III-like protein 1, NTH1, OCTS3, EC 4.2.99.18


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