Anti -NFkBIa (Nuclear Factor kappa B Inhibitor alpha, IkappaB alpha, I(Kappa)B(alpha), IKB
alpha, IKBA, MAD-3, Major Histocompatibility Complex Enhancer Binding Protein MAD3, NFkB
Inhibitor alpha, Nuclear Factor of kappa Light Chain Gene Enhancer in B cells, Nu
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Rabbit |
Supernatant |
B IP IH IC |
|
| The NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkappaB-alpha protein (1-4). In response to many different NF-kappaB-inducing agents—including T-cell mitogens, proinflammatory cytokines, and viral transactivators—the inhibitory IkappaB-alpha is rapidly phosphorylated and degraded (2). Phosphorylation of IkappaB-alpha at Ser32 and Ser36 has been shown to stimulate conjugation with ubiquitin and subsequent degradation of IkappaB-alpha (3). Phosphorylation of IkappaB-alpha at Ser32 and Ser36 is critical for activation of NF-kappaB, which then translocates to the nucleus and induces transcription of genes that protect organism (1-4). | | | Catalog # | N2306-60C | | Applications | Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. | | Recommended Dilution | Western Blot: 1:10,000 | | Immunohistochemistry:1:50-1:100 | | Immunocytochemistry: 1:100 | | Immunoprecipitation: 1:25 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG | | Clone No | 6k217 | | Host | Rabbit | | Source | Human | | Form | Supplied as a liquid. | | Purity | Supernatant | | Immunogen | A synthetic peptide corresponding to human IkappaB-alpha | | Specificity | Recognizes human IkappaB-alpha | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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