Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Molecular Biology » Growth Factors-NGF » Nerve Growth Factor, beta, Recombinant, Mouse (NGFb)

Nerve Growth Factor, beta, Recombinant, Mouse (NGFb)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Nerve Growth Factor is the first of a series of neurotrophic factors that were found to influence the growth and differentiation of sympathetic and sensory neurons. It is comprised of alpha, beta, and gamma subunits. The beta subunit is responsible for its growth stimulating activity. The synthesis of NGF in astrocytes is enhanced by various other cytokines: IL-1, TNF-alpha, PDGF and TGF-beta. Mouse beta-NGF is a noncovalently disulfide-linked homodimer consisting of two 13.4kD polypeptide monomers (240 total amino acid residues). The three disulfide bonds are required for biological activity.
Catalog #N2350-08N
Endotoxin <0.01ng/ug or <0.1EU/ug as per LAL
AA SequenceMSSTHPVFHM GEFSVCDSVS VWVGDKTTAT DIKGKEVTVL AEVNINNSVF RQYFFETKCR ASNPVESGCR GIDSKHWNSY CTTTHTFVKA LTTDEKQAAW RFIRIDTACV CVLSRKATRR G
Biological Activity b-NGF is fully biologically active when compared to standards. Activity was Measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1, the ED50 for this effect is typically 0.3-1ng/ml corresponding to a Specific Activity of 1x106 IU/mg
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight13.4kD
SourceRecombinant, murine, E. coli
Purity98% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE.
FormSupplied as a lyophilized powder. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml, which can be further diluted into other aqueous solutions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links