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You are here:Home » Molecular Biology » Growth Factors-NGF » Nerve Growth Factor, pro, Recombinant, Human (NGF)

Nerve Growth Factor, pro, Recombinant, Human (NGF)

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Specifications

ProNGF is the pro-form of the neurotrophin nerve growth factor. Like the mature protein proNGF is characterized by the cysteine knot motif consisting of three cysteine bridges. The protein predominantly exists as a non-covalently linked homodimer. Recombinant Human Pro-NGF produced in E.coli is a non-glycosylated, polypeptide chain containing 222 amino acids and having a molecular mass of 49,738 Dalton.
Recombinant Pro-NGF is purified by proprietary chromatographic techniques.
Catalog #N2350-09H
Amino Acid SequenceMEPHSESNVPAGHTIPQVHWTKLQHSLDTALRRARSAPAAAIAARVAGQTRNITVDPRLFKKRRLRSPRVL
FSTQPPREAADTQDLDFEVGGAAPFNRTHRSKRSSSHPIFHRGEFSVCDSVSVWVGDKTTATDIKGKEVM
VLGEVNINNSVFKQYFFETKCRDPNPVDSGCRGIDSKHWNSYCTTTHTFVKALTMDGKQAAWRFIRIDTA
CVCVLSRKAVR
Biological ActivityThe activity of the protein can by measured by its stimulating effect on the proliferation of TF1 cells (Chevalier et al. 1994 Blood 83, 1479-85).
EC50 130 ± 30 pM (TF1 cell assay)
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight49.74kD
SourceRecombinant protein corresponding to 222aa from human ProNGF expressed in E. coli
Purity98% as determined by SDS-PAGE
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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