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You are here:Home » Antibodies » Abs to Receptors, Cholinergic » Anti -Nicotinic Acetylcholine Receptor (nAChR)

Anti -Nicotinic Acetylcholine Receptor (nAChR)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites B IP IH
Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine. The nAChRs fall into two subclasses, the muscle-type and the neuronal-type. The muscle-type receptor is a pentameric glycoprotein of five membrane spanning subunits, (2 alpha 1ís, beta 1, gamma or epsilon and delta) which form a ligand gated ion channel. The neuronal nAChR is also a pentamer, but unlike the muscle-type, is made up of only two general types of subunits, alpha and beta of which multiple subtypes have been described (alpha 2 to alpha 8 and beta 2 to beta 4).
Catalog #N2562
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:5000; Detects a 60kD protein representing the gamma subunit and a 65kD protein representing the delta subunit from torpedo skeletal muscle homogenates.
Under non-reducing conditions the delta subunit migrates mostly as a dimer of ~130kD.
Immunohistochemistry: (Frozen sections) 1:1000. Immunohistochemical staining of nAChR in rat skeletal muscle results in strong staining of the motor endplate. Cryostat or permeabilization of the sections is recommended due to the reactivity of this antibody with the cytoplasmic side of the receptor.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No4i285
HostMouse
ConcentrationNot determined
FormSupplied as a liquid in PBS with 0.05% sodium azide.
PurityAscites.
ImmunogenPurified Torpedo californica acetylcholine receptor.
SpecificityDetects nicotinic acetylcholine receptor (nAChR) gamma and delta subunits in torpedo and the delta subunit. Does not detect the alpha 1 and beta 1 subunits. Species Crossreactivity: Rat, Mouse, Chicken, Fish and Amphibians.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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