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You are here:Home » Antibodies » Abs to Receptors, Cholinergic » Anti -Nicotinic Receptor, alpha 4, CT, aa620-627

Anti -Nicotinic Receptor, alpha 4, CT, aa620-627

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IC
Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine. The nAChRs fall into two subclasses, the muscle-type and the neuronal-type. The muscle-type receptor is a pentameric glycoprotein of five membrane spanning subunits, (2 alpha 1ís, beta 1, gamma or epsilon and delta) which form a ligand gated ion channel. The neuronal nAChR is also a pentamer, but unlike the muscle-type, is made up of only two general types of subunits, alpha and beta of which multiple subtypes have been described (alpha 2 to alpha 8 and beta 2 to beta 4).
Catalog #N2565-20
ApplicationsSuitable for the detection of COOH terminal of nicotinic alpha 4 receptor, Western Blot, ELISA, and Immunocytochemistry. Other applications not tested.
Recommended DilutionsImmunocytochemistry (ABC): 1:200
Western Blot: 1:800 detects a single band at 75kD
Optimal dilution determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 10mg/ml BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a lyophilized powder. Reconstitute with 100ul sterile PBS, 10mg/ml BSA. Dilute further with PBS.
PuritySerum
ImmunogenPeptide analogue of the carboxyl terminal of the human nicotinic alpha 4 receptor attached to a carrier protein.
SpecificityRecognizes human nicotinic alpha 4 receptor. Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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