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You are here:Home » Antibodies » Abs to Receptors, Cholinergic » Anti -Nicotinic Receptor, alpha 4

Anti -Nicotinic Receptor, alpha 4

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B IC
Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine. The nAChRs fall into two subclasses, the muscle-type and the neuronal-type. The muscle-type receptor is a pentameric glycoprotein of five membrane spanning subunits, (2 alpha 1ís, beta 1, gamma or epsilon and delta) which form a ligand gated ion channel. The neuronal nAChR is also a pentamer, but unlike the muscle-type, is made up of only two general types of subunits, alpha and beta of which multiple subtypes have been described (alpha 2 to alpha 8 and beta 2 to beta 4).
Catalog #N2565-21
ApplicationsSuitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:800
Immunocytochemistry: 1:200
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
ConcentrationNot determined
FormSupplied as a lyophilized powder. Reconstitution with 0.1ml of 10mg/ml BSA in PBS.
PuritySerum
ImmunogenPeptide analog of the carboxyl terminal of the nicotinic alpha4 receptor (residues #620-627) attached onto a carrier protein.
SpecificityRecognizes Nicotinic alpha4 receptor (620-627) and the reactivity of Nicotinic alpha4 receptor is ~80%. Does not react to Nicotinic alpha3 receptor, Nicotinic alpha5 receptor, Nicotinic alpha7 receptor, Nicotinic beta2 receptor, Nicotinic beta3 receptor and Nicotinic beta4 receptor. Specific for the carboxyl terminal of the nicotinic alpha4 receptor.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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