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You are here:Home » Antibodies » Abs to Receptors, Cholinergic » Anti -Nicotinic Receptor, alpha 5

Anti -Nicotinic Receptor, alpha 5

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B IC
Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine. The nAChRs fall into two subclasses, the muscle-type and the neuronal-type. The muscle-type receptor is a pentameric glycoprotein of five membrane spanning subunits, (2 alpha 1ís, beta 1, gamma or epsilon and delta) which form a ligand gated ion channel. The neuronal nAChR is also a pentamer, but unlike the muscle-type, is made up of only two general types of subunits, alpha and beta of which multiple subtypes have been described (alpha 2 to alpha 8 and beta 2 to beta 4).
Catalog #N2565-25B
ApplicationsSuitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 20ug/ml
Immunocytochemistry (ABC): 40ug/ml. Cells should be fixed using PLP fixative.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~15mg/ml
FormSupplied as a liquid in PBS, pH 7.2.
PurityPurified by precipitation, dialysis and chromatography.
ImmunogenSynthetic peptide corresponding to aa460-468 at the carboxyl terminal of the nicotinic alpha5 receptor, conjugated to a carrier protein.
SpecificityRecognizes Nicotinic alpha5 receptor (aa460-468) at ~75kD and the reactivity of Nicotinic alpha5 receptor is ~95%. Does not react to Nicotinic alpha3 receptor, Nicotinic alpha4 receptor, Nicotinic alpha7 receptor, Nicotinic beta2 receptor, Nicotinic beta3 receptor and Nicotinic beta4 receptor. Specific for the COOH terminal of nicotinic alpha5 receptor.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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