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You are here:Home » Antibodies » Abs to Receptors, Cholinergic » Anti -Nicotinic Receptor, beta 2, CT

Anti -Nicotinic Receptor, beta 2, CT

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Highly Purified E B IC
Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine. The nAChRs fall into two subclasses, the muscle-type and the neuronal-type. The muscle-type receptor is a pentameric glycoprotein of five membrane spanning subunits, (2 alpha 1ís, beta 1, gamma or epsilon and delta) which form a ligand gated ion channel. The neuronal nAChR is also a pentamer, but unlike the muscle-type, is made up of only two general types of subunits, alpha and beta of which multiple subtypes have been described (alpha 2 to alpha 8 and beta 2 to beta 4).
Catalog #N2565-30
ApplicationsSuitable for use in Western Blot, ELISA and Cell Staining techniques. Other applications have not been tested.
Recommended DilutionsELISA: 20ug/ml. Stains fixed cells knoown to express the Nicotinic beta 2 receptor using ABC techniques.
Western Blot: 25ug/ml. Using tissue homogenates and yields a band at ~60kD.
Optimal dilutions to be determined by the researcher.
Control PeptideN2565-44: Nicotinic Receptor, beta 2, Cys492-493-502
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS,10mg/ml BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~21.5mg/ml
FormSupplied as a lyophilized powder. Reconstitute with 100ul of PBS,10mg/ml BSA. No preservatives added.
PurityPurified by precipitation, dialysis and gel filtration chromatography.
ImmunogenPeptide analogue, HSDHSAPSSK of the carboxyl terminal of the nicotinic beta 2 receptor attached to a carrier protein.
SpecificityRecognizes the C-terminus of nicotinic beta 2 receptor. Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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