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You are here:Home » Antibodies » Abs to Receptors, Cholinergic » Anti -Nicotinic Acetylcholine Receptor beta 4 (cholinergic receptor, nicotinic, beta 4 Precursor)

Anti -Nicotinic Acetylcholine Receptor beta 4 (cholinergic receptor, nicotinic, beta 4
Precursor)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Nicotinic acetylcholine receptors, or nAChRs, are cholinergic receptors that form ligand-gated ion channels in the plasma membranes of certain neurons. nAChR is triggered by the binding of the neurotransmitter acetylcholine (ACh) and is also opened by nicotine. After binding ACh, the nAChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. nAChRs are present in many tissues in the body: the neuronal receptors are present in the central nervous system and the peripheral nervous system; and the neuromuscular receptors are found in the neuromuscular junctions of somatic muscles. Neuronal AChR is composed of two different types of subunits: alpha (a) and beta (b).
Catalog #N2565-33C
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:10,000. Detects Nicotinic Acetylcholine Receptor beta 4 in 10ug of rat brain lysate. Rat brain lysate was resolved by electrophoresis, transferred to PVDF and probed with N2565-33C. Proteins were visualized using IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-46E IgG, H&L (HRP) Pab DkxRb
I1904-46K IgG, H&L, X-Adsorbed (HRP) Pab DkxRb
Positive ControlRat brain lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide selected from residues within the Nicotinic Acetylcholine Receptor b4 conjugated to KLH. Epitope: Extracellular Domain
SpecificityRecognizes rat Nicotinic Acetylcholine Receptor beta 4 at ~68kD. Species Crossreactivity: mouse and human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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