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Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified B

NIFK is a nucleolar protein that interacts with Ki67, a protein strongly associated with, and a specific marker of, cell proliferation. The marsupial counterpart of human NIFK was recently identified and suggests that NIFK may be involved in the organization of higher order chromatin structure. NIFK was found to interact with the forkhead associated domain of Ki67 in mitotic cells and the interaction is thought to be phosphorylation-dependent.
Catalog #	N2566-75B
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1:500-1:1000
	observed size: 34 kD
	positive control: NIH3T3 whole cell lysate
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	As reported
Form	Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Purity	Purified
Immunogen	Synthetic peptide corresponding to amino acids 75-90 of mouse NIFK.
Specificity	Recognizes mouse NIFK, Mouse, aa75-90 (Ki67ip)
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.