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N2567-05 NIK, Recombinant, Human, GST-Tag (NF-kappa-beta-inducing Kinase, HsNIK, FTDCR1B, HS, Mitogen-activated Protein Kinase Kinase Kinase 14, MAP3K14, Serine/threonine Protein Kinase NIK) CAS:

Specifications
References
Grade
Highly Purified
Swiss Prot
Q99558
Accession Number
NM_003954
Molecular Weight
96
EU Commodity Code
30021019
Shipping Temp
Dry Ice
Storage Temp
-70°C
EC=2.7.11.25, NFkB-inducing Kinase

Human MAP3K14 (mitogen-activated protein kinase kinase kinase 14; NIK; GenBank Accession No. NM_003954), (a.a. 318-end) with N-terminal GST tag.

Applications
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Source
Baculovirus infected Sf9 cell expression system
Purity
≥65% Purified by glutathione affinity column, followed by a superdex 200 gel filtration coulumn.
Concentration
~0.1mg/ml
Form
Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 3mM DTT, 10% glycerol.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Nadiminty,N. et al., Biochem. Biophys. Res. Commun. 359 (2), 379-384 (2007). 2. Bode,L., Murch,S. and Freeze,H.H., J. Biol. Chem. 281 (12), 7809-7815 (2006).
USBio References
No references available
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