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Specifications

p-NPP is a single component reagent system for microwell assay of Calf Intestine Alkaline Phosphatase (CIAP or CAP) labeled probes and is an excellent substrate for phosphatase based ELISA assays.
Catalog #	N2600
	p-NPP produces a soluble end product which is yellow in color and read at 405-410 nm. Both kinetic and end point determinations can be performed.
	p-NPP is the customary phosphate ester used as a substrate for measuring AP activity due to its high solubility in alkaline buffers which affords zero order kinetics and significant molar extinction coefficient of 18600 mol-1cm-1.
Storage and Stability	Stable for 12 months at 4°C.
Also Available	N2600: p-Nitrophenyl Phosphate (pNPP)
	N2600-10: p-Nitrophenyl Phosphate (pNPP), 50X Concentrate, 0.19M/Liter
Features
• Single component system
• Exhibits low background levels
• Safe to use - no weighing of hazardous powders
• Save preparation time - use right out of the bottle
• Ultra-low sensitivity
• Minimal lot-to-lot variation
Color	Colorless to slightly yellow
Clarity	Clear
Form	Supplied as a liquid in 0.95M DEA, pH 9.0
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	p-Nitrophenylphosphate; Staining System for AP; Soluble