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You are here:Home » BioSeparation? » BioSep-Assay Detection, Substrates » p-Nitrophenyl Phosphate (pNPP)

p-Nitrophenyl Phosphate (pNPP)

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Specifications

p-NPP is a single component reagent system for microwell assay of Calf Intestine Alkaline Phosphatase (CIAP or CAP) labeled probes and is an excellent substrate for phosphatase based ELISA assays.
Catalog #N2600
p-NPP produces a soluble end product which is yellow in color and read at 405-410 nm. Both kinetic and end point determinations can be performed.
p-NPP is the customary phosphate ester used as a substrate for measuring AP activity due to its high solubility in alkaline buffers which affords zero order kinetics and significant molar extinction coefficient of 18600 mol-1cm-1.
Storage and StabilityStable for 12 months at 4°C.
Also AvailableN2600: p-Nitrophenyl Phosphate (pNPP)
N2600-10: p-Nitrophenyl Phosphate (pNPP), 50X Concentrate, 0.19M/Liter
Features
Single component system
Exhibits low background levels
Safe to use - no weighing of hazardous powders
Save preparation time - use right out of the bottle
Ultra-low sensitivity
Minimal lot-to-lot variation
ColorColorless to slightly yellow
ClarityClear
FormSupplied as a liquid in 0.95M DEA, pH 9.0
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesp-Nitrophenylphosphate; Staining System for AP; Soluble


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