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You are here:Home » Antibodies » Abs to Nitrotyrosine » Anti -Nitrotyrosine (nTyr) (Agarose)

Anti -Nitrotyrosine (nTyr) (Agarose)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates. Nitrotyrosine can be detected and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
Catalog #N2700
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionImmunoprecipitation: 40ul of a 50% gel slurry immunoprecipitated nitrated proteins from N2705 as detected by subsequent Western Blot analysis using 1ug/ml N2700-06.
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies I1904-39 IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No0.T.167
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid suspension in PBS, pH 7.4, 0.05% sodium azidewith agrose beads
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified, nitrated Keyhole Limpet Hemocyanin (KLH)
SpecificityRecognizes Nitrotyrosine (nTyr).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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