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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IC

Nitric oxide (NO) is implicated in carcinogenesis(1), chronic infection, inflammation (2) and neurodegeneration (3). High levels of both superoxide and nitric oxide in these tissues interact to form peroxynitrite, a potent oxidant that can modify Tyr residues in proteins to form 3-nitro-tyrosine (4). Tyrosine nitration of mitochondrial manganese superoxide dismutase results in loss of enzymatic activity (4). The nitration of p53 at Tyr residues abolishes its capacity for binding to its DNA consensus sequence (5).
Catalog #	N2700-01
Applications	Suitable for use in ELISA, Western Blot and Immunocytochemistry (ABC detection method). Other applications not tested.
Recommended Dilution	Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
	Immunocytochemistry (ABC): 1:50
	ELISA: 1:1000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Concentration	~0.055mg/ml
Form	Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Synthetic nitro-tyrosine containing peptides coupled to KLH.
Specificity	Detects proteins and peptides containing nitro-tyrosine in a manner independent of the surrounding amino acid sequence. It is a valuable tool for identifying new nitrated proteins as well as for assaying protein nitration and measuring levels of nitrated proteins in tissues and samples. Does not crossreact with unmodified tyrosine or with phospho-tyrosine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.