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You are here:Home » Antibodies » Abs to Nitrotyrosine » Anti -Nitrotyrosine (nTyr)

Anti -Nitrotyrosine (nTyr)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Nitric oxide (NO) and reacti04.10.07ve oxygen species (ROS) are important mediators that produce harmful or protective functions. NO can react with superoxide anions (O2-), yielding the toxic oxidizing agent peroxynitrite (ONOO-). Peroxynitrite induces nitration of tyrosine residues leading to changes in protein structure and function, and alterations in signaling pathways. Antibodies to nitrotyrosine have been used to monitor the status of peroxynitrite-induced modification of proteins.
Catalog #N2700-02
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-5ug/ml
ELISA: 1:10,000-1:50,000 using 50-100ng control nitrated proteins/well.
Optimal dilutions to be determined by the researcher.
Western Blot Nitrated MW Standards N2700-10
Positive Control for Western BlotProteins extracted from human A431 cells or other cells pretreated with peroxynitrite for 5 min
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
Concentration~1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.1% BSA, 0.1% sodium azide, 40% glycerol.
PurityPurified by Immunoaffinity chromatography.
ImmunogenNitrated KLH
SpecificityRecognizes nitrated proteins from all species.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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