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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum E B IP

The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques. Nitrotyrosine is formed in tissues in the presence of the active metabolite NO. Various pathways including the formation of peroxynitrite lead to nitrotyrosine production. It is still unknown whether nitration is merely a footprint of oxidative stress, an important pathway of nitric oxide metabolism or a part of integral processes for maintaining cellular homeostasis.
Catalog #	N2700-02A
Applications	Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution	ELISA: 1:5000-1:20,000
	Western Blot: 1:200-1:2000
	Immunoprecipitation: 1:20-1:100
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	Not determined
Form	Supplied as a lyophilized powder. No preservatives added. Reconstitute in 200ul sterile PBS.
Purity	Serum
Immunogen	Nitrotyrosine (KLH)
Specificity	Recognizes free and bound nitrotyrosine. Does not react with free or bound tyrosine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.