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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified E B IH

The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques. Nitrotyrosine is formed in tissues in presence of the active metabolite NO. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production.
Catalog #	N2700-02C
Applications	Suitable for use in ELISA, Western Blot and Immunohistochemistry (Fr) (paraffin-embedded sections). Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	9A40
Host	Mouse
Concentration	As reported
Form	Supplied as a liquid in PBS, pH 7.2.
Purity	Purified
Specificity	This highly specific monoclonal antibody reacts with nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine. The antibody does not recognize other antigens with a nitrotyrosine-like structure such as tyrosine, 3-chlorotyrosine and phenylalanine, illustrating the specificity of the antibody. This specificity has been described in the mentioned article of Jessica ter Steege.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.