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You are here:Home » Antibodies » Abs to Nitrotyrosine » Anti -Nitrotyrosine (nTyr)

Anti -Nitrotyrosine (nTyr)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Nitrotyrosine is formed in tissues in presence of the active metabolite NO and is a stable end product of nitrosylation of tyrosine. Inflammation is characterized by increased nitric oxide (NO) production. NO reacts rapidly with superoxide to form peroxynitrite. At physiological pH and in the presence of transition metals, peroxynitrite undergoes heterolytic cleavage to form hydroxyl anion and nitronium ion, the latter of which nitrates protein tyrosine residues. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques, Amyotrophic Lateral Sclerosis (ALS) and Multiple Sclerosis (MS). Thus, the presence of nitrotyrosine on proteins can be used as a marker for peroxynitrite formation in vivo and consequently as a marker of NO-mediated tissue damage.
Catalog #N2700-03
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1:50 for reduced and no-reduced samples. Block with 5% BSA or skimmed milk.
Immunohistochemistry (Frozen, cytospins): 1:50 with acetone fixation for 10 minutes at -20 °C. Block endogenous peroxidase with 0.3% H2O2 in PBS (or methanol for intracellular staining). Block with 10% NGS or 5% BSA for 30 minutes.
Immunohistochemistry (Paraffin): 1:200-1:400 with 10% formalin fixation, Use 3% H2O2 to block endogenous peroxidases. Use citrate buffer pH 6.0 for 1 minute at 100°C as antigen retrieval treatment.
Optimal dilutions to be determined by the researcher.
Western Blot ControlsMouse kidney lysate, mouse optic nerve, retina , spinal cord and brain lysates, rat aorta lysate
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG2b
Clone No6A610
HostMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
PurityPurified
ImmunogenNitrated KLH
SpecificityRecognizes nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine. Does not crossreact with Phosphotyrosine or Chlorotyrosine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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