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You are here:Home » Antibodies » Abs to Nitrotyrosine » Anti -Nitrotyrosine (nTyr) (Biotin)

Anti -Nitrotyrosine (nTyr) (Biotin)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques. Nitrotyrosine is formed in tissues in presence of the active metabolite NO and is a stable end product of nitrosylation of tyrosine. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production.
Catalog #N2700-03A
ApplicationsCan be used for Immunohistology on paraffin sections and frozen sections. Furthermore the antibody is useful for Western Blot and for immunoassay.
Recommended DilutionImmunohistology: 1:10
Western Blot: 1:10
Warning: keep sterile, do not add sodium azide or any nitric containing preservative since this will affect the antibody.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No6A610
HostMouse
Concentration~0.1mg/ml
ImmunogenNitrated KLH
FormSupplied as a liquid in PBS, containing 0.1% BSA. Conjugated to Biotin.
PurityPurified IgG
SpecificityReacts with nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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