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Specifications

Clone Host Grade Applications Polyclonal Goat Serum E B

The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates. Nitrotyrosine can be detected and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
Catalog #	N2700-07
Applications	Suitable for use in detection of nitrated tyrosine, ELISA and Immunoblot. Other applications have not been tested.
Recommended Dilution	ELISA: 1:20,000-1:160,000
	Western Blot: 1:20,000-1:160,000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Goat
Concentration	Not determined
Form	Supplied as liquid with 0.1% sodium azide.
Purity	Neat serum
Immunogen	Purified, nitrated Keyhole Limpet Hemocyanin (KLH)
Specificity	Specifically binds to nitro-LDL and other nitrated proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.