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Specifications

Clone Host Grade Applications Polyclonal Goat Purified E B IH

The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates. Nitrotyrosine can be detected and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
Catalog #	N2700-07A
Applications	Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Immunohistochemistry: 1:100-1:10,000
	Western Blot:1:1000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Purity	Purified
Immunogen	Nitrated KLH
Specificity	Recognizes free Nitrotyrosine and nitrotyrosine-containing proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.