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You are here:Home » Antibodies » Antibodies-Nitrotyrosine » Anti -Nitrotyrosine (nTyr)

Anti -Nitrotyrosine (nTyr)


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Clone Host Grade Applications
Polyclonal Goat Purified E B IH
The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates. Nitrotyrosine can be detected and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
Catalog #N2700-07A
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000
Immunohistochemistry: 1:100-1:10,000
Optimal dilutions to be determined by the researcher.
Positive ControlN2705-01: Nitrotyrosine, Immunoblot Positive Control (nTyr)
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
FormSupplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
ImmunogenNitrated KLH
SpecificityRecognizes free Nitrotyrosine and nitrotyrosine-containing proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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