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You are here:Home » Antibodies » Abs to Nitrotyrosine » Anti -Nitrotyrosine (nTyr)

Anti -Nitrotyrosine (nTyr)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH
The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques. Nitrotyrosine is formed in tissues in the presence of the active metabolite NO. Various pathways including the formation of peroxynitrite lead to nitrotyrosine production. It is still unknown whether nitration is merely a footprint of oxidative stress, an important pathway of nitric oxide metabolism or a part of integral processes for maintaining cellular homeostasis.
Catalog #N2700-09
ApplicationsSuitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 0.5-5ug/ml
Western Blot: 1-2ug/ml
Immunoprecipitation: ~5ug/IP reaction
Immunohistochemistry (Frozen, paraffin): 1-2ug/ml; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive ControlWestern Blot: Peroxynitrite treated A431 cells lysates
Immunohistochemistry: Human pancreas tissue
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3G210
HostMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenNitrotyrosine
SpecificityRecognizes nitrotyrosine as both free amino acid and as present in nitrated proteins. Reactivity of this antibody with nitrated proteins is independent of the protein species of origin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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