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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Nitric oxide (NO) and reactive oxygen species (ROS) are important mediators to produce harmful or protective functions. NO can react with superoxide anions (O2-), yielding the toxic oxidizing agent peroxynitrite (ONOO-). Peroxynitrite induces nitration of tyrosine residues leading to changes of protein structure and function, and alterations in signaling pathways. Antibodies to nitrotyrosine have been used to monitor the status of peroxynitrite-induced modification of proteins.
Catalog #	N2700-11
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1-5ug/ml. Proteins extracted from human A431 cells or other cells pretreated with peroxynitrite for 5 min may serve as good positive control.
	ELISA: 1:10,000-1:50,000 using 50-100ng N2700-10 Nitrated MW Standards, Western Blot/well.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3H37
Host	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, BSA, 0.025% sodium azide.
Purity	Purified by Protein A/G affinity chromatography.
Immunogen	Nitrated KLH
Specificity	Recognizes nitrated proteins from all species.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.