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You are here:Home » Antibodies » Abs to Nitrotyrosine » Anti -Nitrotyrosine (nTyr)

Anti -Nitrotyrosine (nTyr)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Serum E B
Nitric oxide (NO) and reactive oxygen species (ROS) are important mediators to produce harmful or protective functions. NO can react with superoxide anions (O2.-), yielding the toxic oxidizing agent peroxynitrite (ONOO-). Peroxynitrite induces nitration of tyrosine residues leading to changes of protein structure and function, and alterations in signaling pathways. Antibodies to nitrotyrosine have been used to monitor the status of peroxynitrite-induced modification of proteins.
Catalog #N2700-12
ApplicationsSuitable for use inELISA, Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-5ug/ml. Proteins extracted from human A431 cells or other cells pretreated with peroxynitrite for 5 min may serve as good positive control for Western.
ELISA: 1:10,000-1:50,000 using 50-100ng control nitrated proteins/well.
Optimal dilutions to be determined by the researcher.
Western Blot Nitrated MW Standards N2700-10
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
ConcentrationAs reported
FormSupplied as a liquid, neat serum, 0.1% sodium azide, 40% glycerol.
PuritySerum
ImmunogenNitrated-KLH
SpecificityAntibodies should react with nitrated proteins from all species
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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