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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B

The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates. Nitrotyrosine can be detected and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.
Catalog #	N2700-12A
Applications	Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilutions	ELISA: 1:200-1:40,000
	Optimal dilution determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, 1mM EDTA, 0.02% sodium azide.
Purity	Purified by Nitrated protein-sepharose affinity chromatography.
Immunogen	Pure nitrated Keyhole Limpet Hemocyanin (NY-KLH)
Specificity	Specifically binds to nitrate LDL and other nitrated proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.