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You are here:Home » Antibodies » Abs to Nitrotyrosine » Anti -Nitrotyrosine (nTyr)

Anti -Nitrotyrosine (nTyr)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E B
The enzymatic conversion of arginine to citrulline by nitric oxide synthase (NO Synthase) leads to the formation of nitric oxide (NO). NO forms peroxynitrite following the reaction with superoxide. Peroxynitrite is a potent oxidant that reacts with a variety of biological molecules, including tyrosine residues in proteins. Tyrosine nitration can inactivate enzymes and receptors that depend on tyrosine residues for their activity. Nitration prevents phosphorylation of tyrosnine residues important for signal transduction.
Catalog #N2700-13D
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsELISA: 0.05ug/ml
Western Blot: 1ug/ml for AP/BCIP/NBT (MTT detection) or 0.1ug/ml for HRPO/ECL detection.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6D609
HostMouse
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder from PBS, PEG, sucrose, 0.1% sodium azide. Reconstitute in 1ml sterile ddH2O.
PurityPurified by thiophilic adsorption and size exclusion chromatography.
ImmunogenKLH modified with nitrotyrosine
SpecificityRecognizes Nitrotyrosine (nTyr). Species Crossreactivity: human, mouse, rat and canine
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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