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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IP

Nitrotyrosine arises from the nitration of protein tyrosine residues, by reactive nitrogen species such as peroxynitrite and nitrogen dioxide, and can be used as a measure of NO-dependent oxidative stress and cell damage. Increased levels of nitrotyrosine are detectable in various pathological conditions including atherosclerosis, rheumatoid arthritis, cardiovascular disease, lung cancer and vasculitis.
Catalog #	N2700-16D
Applications	Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilutions	ELISA: 2-100ng/ml
	Western Blot: 0.1ug/ml
	Immunoprecipitation: 1ug/ml
	Optimal dilutions to be determined by the researcher.
Crossreactivity Data	Specificity as determined by ELISA competition experiments.
	Compound InhibitionNitrotyrosine 95%Nitrotryptophan 4%Chlorotyrosine 3%Phosphotyrosine 2%Tyrosine 0% Aminotyrosine 0%
Recommended Secondary Antibodies	I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab GtxMo
	I1904-65N: IgG (HRP) Pab Rb xMo
	I1903-07E: IgG (HRP) Pab Rb xMo
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	10A9
Host	Mouse
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Protein-bound Nitrotyrosine
Specificity	Recognizes both free and protein-bound nitrotyrosine (Tyr-NO2), including human serum nitro albumin, nitro insulin and nitro hemoglobin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	Tyr-NO2