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You are here:Home » Antibodies » Abs to Nitrotyrosine » Anti -Nitrotyrosine (nTyr)

Anti -Nitrotyrosine (nTyr)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP
Nitrotyrosine arises from the nitration of protein tyrosine residues, by reactive nitrogen species such as peroxynitrite and nitrogen dioxide, and can be used as a measure of NO-dependent oxidative stress and cell damage. Increased levels of nitrotyrosine are detectable in various pathological conditions including atherosclerosis, rheumatoid arthritis, cardiovascular disease, lung cancer and vasculitis.
Catalog #N2700-16D
ApplicationsSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionsELISA: 2-100ng/ml
Western Blot: 0.1ug/ml
Immunoprecipitation: 1ug/ml
Optimal dilutions to be determined by the researcher.
Crossreactivity DataSpecificity as determined by ELISA competition experiments.
Compound Inhibition Nitrotyrosine 95% Nitrotryptophan 4% Chlorotyrosine 3% Phosphotyrosine 2% Tyrosine 0% Aminotyrosine 0%
Recommended Secondary AntibodiesI1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab GtxMo
I1904-65N: IgG (HRP) Pab Rb xMo
I1903-07E: IgG (HRP) Pab Rb xMo
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No10A9
HostMouse
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenProtein-bound Nitrotyrosine
SpecificityRecognizes both free and protein-bound nitrotyrosine (Tyr-NO2), including human serum nitro albumin, nitro insulin and nitro hemoglobin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesTyr-NO2


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