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Specifications

Clone Host Grade Applications Polyclonal Rabbit Serum E IH IF

Located in the cell membrane of thyroid cells, NIS can allow sodium and iodine flow across the membrane. Suitable for studying the sensitivity of thyroid tumors to iodine treatment..
Catalog #	N2750-10
Applications	Suitable for ELISA, Immunohistochemistry and Immunofluorescence Microscopy. Other applications not tested.
Recommended Dilution	Immunohistochemistry (Frozen sections): 1:1200-1:2000 using FITC conjugates as secondary antibodies; Incubation Time Overnight at 4°C.
	Optimal dilution to be determined by researcher.
Positive Control	Frozen sections of rat thyroid.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Rat
Concentration	Not Determined
Form	Supplied as a lyophilized powder. Reconstitute in 50-100ul of sterile ddH2O. Dilute further with 0.1M PBS, 1% BSA, 0.1% sodium azide.
Purity	Serum
Immunogen	Synthetic peptide from the C-terminus of rat NIS.
Specificity	Recognzies rat NIS. Species Crossreactivity: porcine and human.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.