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You are here:Home » Antibodies » Abs to Proteins » Anti -Nkx3.1 (NK-2 Family Homeodomain Transcription Factor)

Anti -Nkx3.1 (NK-2 Family Homeodomain Transcription Factor)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
The Nkx3.1 protein is the product of a prostate-specific regulatory gene known as the NKX3.1 homeobox gene. NKX3.1 maps to chromosome band 8p21. Loss of heterozygosity at this particular site has been associated with tissue dedifferentiation and loss of androgen responsiveness in the progression of prostate cancer. Loss of specific regions of chromosome 8 occur in 60-80% of prostate tumors. Although a broad range of tumor suppressor genes, including NKX3.1, PTEN, Rb, p27, p16, and Myc, have been proposed as the genetic loci directly responsible for the chromosomal abnormalities characteristic of prostate cancer, none of these genes has consistently demonstrated mutations in a large population of prostate cancer specimens. Mouse studies of NKX3.1 have demonstrated that the gene is essential for normal prostate morphogenesis and function. Inactivation produces prostatic epithelial hyperplasia and dysplasia, closely modeling a preneoplastic condition. In vitro, overexpression of human and mouse NKX3.1 suppresses growth and tumorigenicity of prostate carcinoma cells. These findings suggest that NKX3.1 may be responsible for maintaining the normal prostate, and its loss may represent a predisposing event for prostate carcinogenesis.
Catalog #N2850-30B
ApplicationsSuitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry (Frozen and paraffin): 10-20ug/ml
Western Blot: 1-3ug/ml
ELISA: 0.1-1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6A141
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A chromatography
ImmunogenFusion protein containing the N-terminal domain of the human Nkx3.1 protein.
SpecificityRecognizes human Nkx3.1 protein at ~34kD. Does not crossreact with mouse. Reactivity has been confirmed with human LN Cap-FGC prostate carcinoma cell lysates.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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