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You are here:Home » Antibodies » Abs to Transcription Factors, Homeobox (HOX) » Anti -Nkx6.1 (NK Homeobox Factor 6.1, NKX6A)

Anti -Nkx6.1 (NK Homeobox Factor 6.1, NKX6A)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
NKX6.1 (NK homeobox factor 6.1; also NKX6A) is a 39-46kD member of the homeodomain family of transcription factors. It is expressed in embryonic ventral neural tube where it drives neural progenitors to form motoneurons and V2-type interneurons. It is also expressed in both fetal and neonatal pancreas where it promotes a b-cell phenotype. NKX6.1 exhibits both transcriptional gene repression (NKX6.2 and Dbx2) and activation (cyclin A2 and B1). When overexpressed, it induces b-cell mitosis. Human NKX6.1 is 367aa in length. It contains a repressor region composed of Pro and Ala (aa136-173), a DNA binding homeobox (aa236-295) and a Glu:Asp-rich transactivation domain (aa305-337). Over aa1-120, human NKX6.1 shares 96% aa identity with mouse NKX6.1.
Catalog #N2850-33
ApplicationsSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml using bTC-6 mouse beta cell insulinoma cell line.
Immunohistochemistry (Paraffin): 5-15ug/ml detects NKX6.1 in immersion fixed paraffin-embedded human pancreas.
Immunohistochemistry (Frozen): 5-15ug/ml detects NKX6.1 in perfusion fixed frozen sections of rat pancreas.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C after receipt. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa1-120 of human NKX6.1 expressed in E. coli (NP_006159).
SpecificityRecognizes human NKX6.1. Species Crossreactivity: mouse, rat. Shows less than 1% crossreactivity with recombinant human NKX3.1 in direct ELISA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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