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You are here:Home » Antibodies » Abs to Transcription Factors, Homeobox (HOX) » Anti -Nkx6.1 (NK Homeobox Factor 6.1, NKX6A)

Anti -Nkx6.1 (NK Homeobox Factor 6.1, NKX6A)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IC
Nkx6.1 (NK homeobox factor 6.1; also NKX6A) is a 39-43kD member of the homeodomain family of transcription factors. It is expressed in embryonic ventral neural tube where it drives neural progenitors to form motoneurons and V2-type interneurons. It is also expressed in both fetal and neonatal pancreas where it promotes a b-cell phenotype. Nkx6.1 exhibits both transcriptional gene repression (Nkx6.2 and Dbx2) and activation (cyclin A2 and B1). When overexpressed, it induces b cell mitosis. Human Nkx6.1 is 367aa in length. It contains a repressor region composed of Pro and Ala (aa136-173), a DNA binding homeobox (aa236-295) and a Glu:Asp-rich transactivation domain (aa305-337). Over (aa1-120), human Nkx6.1 shares 96% aa identity with mouse Nkx6.1.
Catalog #N2850-34
ApplicationsSuitable for use in Direct ELISA and Immunocytochemistry. Other applications not tested.
Recommended DilutionsDirect ELISA: 0.5-1ug/ml. The detection limit is ~5ng/well.
Immunocytochemistry: 10ug/ml detected human Nkx6.1 in betaTC6 cells. Cells were fixed with PBS, 4% paraformaldehyde for 20 minutes at RT and blocked in PBS, 10% normal donkey serum, 0.3% Triton X-100, 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted N2850-34 for 3 hours at RT. Between each step, cells were washed with PBS, 0.1% BSA.
Optimal dilutions to be determined by the researcher.
HybridomaMyeloma cells with B cells from Balb/c mice.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No11D12
HostMouse
SourceHuman
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant corresponding to aa1-120 from human Nkx6.1 expressed in E. coli (Accession #P78426).
SpecificityRecognizes human Nkx6.1. Does not crossreact with recombinant human Nkx2E, 2.2, 3.1 or recombinant mouse Nkx3.1. Species Seqeuence Homology: mouse, 96%
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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