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You are here:Home » Antibodies » Antibodies-Transcription Factors, Homeobox (HOX) » Anti -Nkx6.1 (NK Homeobox Factor 6.1, NKX6A)

Anti -Nkx6.1 (NK Homeobox Factor 6.1, NKX6A)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E IC
Nkx6.1 (NK homeobox factor 6.1; also NKX6A) is a 39-43kD member of the homeodomain family of transcription factors. It is expressed in embryonic ventral neural tube where it drives neural progenitors to form motoneurons and V2-type interneurons. It is also expressed in both fetal and neonatal pancreas where it promotes a b-cell phenotype. Nkx6.1 exhibits both transcriptional gene repression (Nkx6.2 and Dbx2) and activation (cyclin A2 and B1). When overexpressed, it induces b cell mitosis. Human Nkx6.1 is 367aa in length. It contains a repressor region composed of Pro and Ala (aa136-173), a DNA binding homeobox (aa236-295) and a Glu:Asp-rich transactivation domain (aa305-337). Over (aa1-120), human Nkx6.1 shares 96% aa identity with mouse Nkx6.1.
Catalog #N2850-34
ApplicationsSuitable for use in Direct ELISA and Immunocytochemistry. Other applications not tested.
Recommended DilutionsDirect ELISA: 0.5-1ug/ml. The detection limit is ~5ng/well.
Immunocytochemistry: 10ug/ml detected human Nkx6.1 in betaTC6 cells. Cells were fixed with PBS, 4% paraformaldehyde for 20 minutes at RT and blocked in PBS, 10% normal donkey serum, 0.3% Triton X-100, 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted N2850-34 for 3 hours at RT. Between each step, cells were washed with PBS, 0.1% BSA.
Optimal dilutions to be determined by the researcher.
HybridomaMyeloma cells with B cells from Balb/c mice.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No11D12
Concentration~0.5mg/ml (after reconstitution)
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant corresponding to aa1-120 from human Nkx6.1 expressed in E. coli (Accession #P78426).
SpecificityRecognizes human Nkx6.1. Does not crossreact with recombinant human Nkx2E, 2.2, 3.1 or recombinant mouse Nkx3.1. Species Seqeuence Homology: mouse, 96%
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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