| Nkx6.1 (NK homeobox factor 6.1; also NKX6A) is a 39-43kD member of the homeodomain family of transcription factors. It is expressed in embryonic ventral neural tube where it drives neural progenitors to form motoneurons and V2-type interneurons. It is also expressed in both fetal and neonatal pancreas where it promotes a b-cell phenotype. Nkx6.1 exhibits both transcriptional gene repression (Nkx6.2 and Dbx2) and activation (cyclin A2 and B1). When overexpressed, it induces b cell mitosis. Human Nkx6.1 is 367aa in length. It contains a repressor region composed of Pro and Ala (aa136-173), a DNA binding homeobox (aa236-295) and a Glu:Asp-rich transactivation domain (aa305-337). Over (aa1-120), human Nkx6.1 shares 96% aa identity with mouse Nkx6.1. |
|
| Catalog # | N2850-34 |
| Applications | Suitable for use in Direct ELISA and Immunocytochemistry. Other applications not tested. |
| Recommended Dilutions | Direct ELISA: 0.5-1ug/ml. The detection limit is ~5ng/well. |
| Immunocytochemistry: 10ug/ml detected human Nkx6.1 in betaTC6 cells. Cells were fixed with PBS, 4% paraformaldehyde for 20 minutes at RT and blocked in PBS, 10% normal donkey serum, 0.3% Triton X-100, 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted N2850-34 for 3 hours at RT. Between each step, cells were washed with PBS, 0.1% BSA. |
| Optimal dilutions to be determined by the researcher. |
| Hybridoma | Myeloma cells with B cells from Balb/c mice. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2b |
| Clone No | 11D12 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.5mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Recombinant corresponding to aa1-120 from human Nkx6.1 expressed in E. coli (Accession #P78426). |
| Specificity | Recognizes human Nkx6.1. Does not crossreact with recombinant human Nkx2E, 2.2, 3.1 or recombinant mouse Nkx3.1. Species Seqeuence Homology: mouse, 96% |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
