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You are here:Home » Antibodies » Abs to Lectins » Anti -NKG2A/C/E (FITC) (Natural Killer, NK)

Anti -NKG2A/C/E (FITC) (Natural Killer, NK)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified FC
Catalog #N2851-75D
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:10-1:50, Membrane permeabilization is required for this application.
Use 10ul to label 106 cells in 100ul.
Optimal dilution determined by the researcher
HybridomaSpleen cells from immunized Lewis rats were fused with cells of the mouse P3X63-Ag8.653 myeloma cell line.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No6A447
HostRat
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC).
PurityPurified by Protein G affinity chromatography.
ImmunogenCHO transfected cells expressing the B6 allele of NKG2A.
SpecificityRecognizes murine NKG2A, NKG2C and NKG2E, which are isoforms of the NKG2 lectin-like family. In mice, NKG2 subunits associate with CD94 to form heterodimers at the surface of natural killer (NK) cells. The CD94/NKG2 heterodimer is the receptor for a non-classical MHC class I ligand, which is Qa-1 in the mouse. Reported to block ligand binding to the receptor(1).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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