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You are here:Home » Antibodies » Abs to Lectins » Anti -NKG2A/C/E (Low Endotoxin) (Natural Killer, NK)

Anti -NKG2A/C/E (Low Endotoxin) (Natural Killer, NK)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified FC
NKG2A, NKG2C and NKG2E, which are isoforms of the NKG2 lectin-like family. In mice, NKG2 subunits associate with CD94 to form heterodimers at the surface of natural killer (NK) cells. The CD94/NKG2 heterodimer is the receptor for a non-classical MHC class I ligand, which is Qa-1 in the mouse.
Catalog #N2851-75E
ApplicationsSuitable for use in Flow Cytometry and Functional assays. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:12-1:50. Use 20ul to label 10e6 cells in 100ul.
Optimal dilution determined by the researcher.
Endotoxin Level <0.01 EU/ug.
HybridomaSpleen cells from immunized Lewis rats were fused with cells of the mouse P3X63-Ag8.653 myeloma cell line.
Recommeneded Secondary ReagentsF(ab')2 Goat anti rat IgG:FITC (mouse adsorbed)
F(ab')2 Goat anti rat IgG:RPE (mouse adsorbed)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No6A447
HostRat
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, before the addition of glycerol to 40%. No preservatives added.
PurityPurified by Protein G affinity chromatography.
ImmunogenCHO transfected cells expressing the B6 allele of NKG2A.
SpecificityRecognizes murine NKG2A/C/E. Reported to block ligand binding to the receptor (1).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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