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You are here:Home » Antibodies » Abs to Lectins » Anti -NKG2A/CIE (Natural Killer, NK)

Anti -NKG2A/CIE (Natural Killer, NK)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified FC
The CD94/NKG2 heterodimer is the receptor for a non-classical MHC class I ligand, which is Qa-1 in the mouse.
Reported to block ligand binding to the receptor (1).
Catalog #N2851-75H
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionFlow Cytometry: 1:12.5-1:50 Use 20ul to label 10e6 cells in 100ul.
Optimal dilution determined by the researcher
HybridomaSpleen cells from immunized Lewis rats were fused with cells of the mouse P3X63-Ag8.653 myeloma cell line.
Recommended Secondary AntibodiesI1904-49U: IgG, F(abí)2, H&L X-Adsorbed (FITC) RbxRt
I1904-49H: IgG, F(abí)2, H&L X-Adsorbed (HRP) RbxRt
I1904-54B1: IgG, F(abí)2, X-adsorbed (HRP) GtxRt
Recommended Negative ControlI1904-82D1: IgG2a, Rat Negative Control
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No6A447
HostRat
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, before the addition of glycerol to 40.
PurityPurified by Protein G affinity chromatography.
ImmunogenCHO transfected cells expressing the B6 allele of NKG2A.
SpecificityRecognizes mouse NKG2A, NKG2C and NKG2E, which are isoforms of the NKG2 lectin-like family. In mice, NKG2 subunits associate with CD94 to form heterodimers at the surface of natural killer (NK) cells.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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