| The CD94/NKG2 heterodimer is the receptor for a non-classical MHC class I ligand, which is Qa-1 in the mouse. |
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| Reported to block ligand binding to the receptor (1). |
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| Catalog # | N2851-75H |
| Applications | |
| Suitable for use in Flow Cytometry. Other applications not tested. |
| Recommended Dilution | Flow Cytometry: 1:12.5-1:50 Use 20ul to label 10e6 cells in 100ul. |
| Optimal dilution determined by the researcher |
| Hybridoma | Spleen cells from immunized Lewis rats were fused with cells of the mouse P3X63-Ag8.653 myeloma cell line. |
| Recommended Secondary Antibodies | I1904-49U: IgG, F(ab’)2, H&L X-Adsorbed (FITC) RbxRt |
| I1904-49H: IgG, F(ab’)2, H&L X-Adsorbed (HRP) RbxRt |
| I1904-54B1: IgG, F(ab’)2, X-adsorbed (HRP) GtxRt |
| Recommended Negative Control | I1904-82D1: IgG2a, Rat Negative Control |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 6A447 |
| Host | Rat |
| Source | Mouse |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, before the addition of glycerol to 40. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | CHO transfected cells expressing the B6 allele of NKG2A. |
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| Specificity | Recognizes mouse NKG2A, NKG2C and NKG2E, which are isoforms of the NKG2 lectin-like family. In mice, NKG2 subunits associate with CD94 to form heterodimers at the surface of natural killer (NK) cells. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
